Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNFVHKTVNGLKSLLDEKGAIQLFCSEGDIMEFL------VTFSPDKATLNDI----QSFEAKHQLSLPEDYQKFI-TLHN---------GAKIFEILSDGENIGGGLQLFSLEEIEEELKYEDLFEGINGIPIGYLLEECHLMIDKDKINQGDPNYLYIFESGLEYNPLNLNFEIFLDRYILANGEPFWDWRYYTAENYYRTR 2R7S Chain:A ((836-926)) ------------KAKLNSYAPISLEKRRAQISALLTMLQKPVTFKSSKITINDILRDIKPFFTVSDAHLPIQYQKFMPTLPDNVQYIIQCIGSRTYQIEDDGS------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2R7S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 23 1769 76.89 24.91 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 76.89 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_2R7S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R7S-query.scw PDB file : Tito_Scwrl_2R7S.pdb: