Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKRKLGTSDLDISEVGLGCMSLGTEKNKALS---ILDEAIELGINYLDTADLYDR--GRNEEIVGDAIQN----RRHDIILATKAG-NRWDD--GSEGWYWDPSKAYIKEAVKKSLTRLKTDYIDLYQLHGGTIEDNIDETIEAFEELKQEGVIRYYGISSIRPNVIK---EYVKKSNIVSIMMQ--FSLFDRRPEE--WLPLLEEHQISVVARGPVAKGLLTEKPLDQASESMKQNGYLSYSFEELT-NARKAMEEVAPDL--SMTEKSLQYLLAQPAVASVITGASKIEQLRENIQAANARRLTEEEIKALQSHTKQDIYKAHRS 3N6Q Chain:A ((13-331)) MQYRYCGKSGLRLPALSLGLWHNFGHVNALESQRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDMWPGPYGSGG-----SRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSGKALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVGCIAFTPLAQGLLTGKYL----------------LTEANLNSLRLLNEMAQQRGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQH-----------

General information: TITO was launched using: RESULT: Template: 3N6Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1490 60575 40.65 221.07 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 40.65 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3N6Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N6Q-query.scw PDB file : Tito_Scwrl_3N6Q.pdb: