Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMDFNLFMNDIVRQARQEITAAGYTELKTAEEVDEALT-K--KGTTLVMVNSVCGCAGGIARPAAYHSVHYDKRPDQLVTVFAGQDKEATARARDYFEGYPPSSPSFAILKDGKIMKMVER----HEIEGH--EPMAVVAKLQEAFEEYCEEV 2AV4 Chain:A ((22-139)) -----------------------MLQHLNSGWAVDQAIVNEDERLVCIRFGHDYDPD-CMKMDELLYKV-A-DDIKNF--CVIYLVDITEVPDFNTMYEL--YDPVSVMFFYRNKHMMIDLGTGNNNKINWPMNNKQEFIDIVETIFR------

General information: TITO was launched using: RESULT: Template: 2AV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 459 -18277 -39.82 -167.67 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -39.82 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_2AV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AV4-query.scw PDB file : Tito_Scwrl_2AV4.pdb: