Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLVIVIHPNLETSVVNKTWMNRLKQEKDITVHDLYGEYPNFIIDVEKEQQLLLDHERIVFQFPMYWYSSPALLKQWEDDVLTHGWAYGTGG----TKLHGKELLLAISLGAQESDYQAGGEYNITISELIRPFQ-VTANYIGMRFLPAFTQYGTLHLSKEDVKNSAERLVDYLKAEH 3G5M Chain:A ((70-203)) ------------------------------VETHEAYKQR-SLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTG-VNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKG-------------

General information: TITO was launched using: RESULT: Template: 3G5M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 488 -36250 -74.28 -281.01 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -74.28 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_3G5M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G5M-query.scw PDB file : Tito_Scwrl_3G5M.pdb: