Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSDQ-KFIILDKSHEVGESWKDRYDSLVLFTSRMYSSLPGMH------L---EG--EKHGFPSKNEIVAYLKKYVKKFEIPIQLRTEVISVLKIKNYFLIKTNREEYQTKNLVIATGPFHTPNIPSISKDLSDNINQLHSSQYKNSKQLAYGNVLVVGGGNSGAQIAVELSKE-RVTYLACSNKLVYFPLMIGKRSIFWWFDKLGVLHASHTSIVGKFIQKKGDPVFGHELKHAIKQKEIILK--K--RVIAAKQ----NEIIFKDSST-LEVNNIIWATGFRNPL-CWINIKGVLDQEGRIIHH-RG-VSPVEGLYFIGLPWQHKRGSALLQGVGNDAEY--IVKQMNGE 3D1C Chain:A ((4-336)) HHKVAIIGAGAAGIGMAITLKDFGITDVIILEKG-TVGHSFKHWPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYELNIFENTVVTNISADDAYYTIATTTETYHADYIFVATGDYNFPKKPFKY--------GIHYSEIEDFDNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTS----------PSVRLSPYT--------------RQR------------LG-NVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPIVQQLF-VTTNQDIKLTTHDESTRYPNIFMIGATVENDN-AKLCYIYKFRARFAVLAHLLTQ-

General information: TITO was launched using: RESULT: Template: 3D1C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1615 14297 8.85 48.30 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 8.85 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3D1C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D1C-query.scw PDB file : Tito_Scwrl_3D1C.pdb: