Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISTKGRYGLTIMIELAKKHGEGPTSLKSIAQTNNLSEHYLEQLVSPLRNAGLVKSIRGAYGGYVLGSEPDAITAGDIIRVLEGPISPVEVLEDEEPA---KRELWIRIRDAVKEVLDSTTLEDLASYTDGEQEAYMFYI 4CHU Chain:A ((2-131)) -RLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQLFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVD-----------KSLTHALWRDLSDRLTGFLNNITLGELVN-------------

General information: TITO was launched using: RESULT: Template: 4CHU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 403 -60123 -149.19 -532.06 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -149.19 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_4CHU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CHU-query.scw PDB file : Tito_Scwrl_4CHU.pdb: