Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKFEASIDNLKEIEMNAYAYELIREIVLPDMLGQDYSSMMYWAGKHLARKFPLE-SWEEFPAFFEEAGWGTL-----TNVSA-KKQELEFELEGPIISNRLKH-QKEPCFQLEAGFIAEQIQLMNDQIAESYEQVKKRADKVVLTVKWDMKDPV 2BJN Chain:A ((36-159)) ---------------------------------------TKLENMGFRVGQGLIERF-ELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQM--EHASKYLAFTCGLIRGGLSN-LGIKSIVTAEVSS-MPACKFQVMIQK----

General information: TITO was launched using: RESULT: Template: 2BJN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 372 6338 17.04 63.38 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 17.04 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_2BJN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BJN-query.scw PDB file : Tito_Scwrl_2BJN.pdb: