Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKKTWKRFLHFSSAALAAGLIFTSAAPAEAAFWGASNELLHDPTMIKEGSSWYALGTGLTEERGLRVLKSSDAKNWTVQKSIFTTPLSWWSNYVPNYGQNQWAPDIQYYNGKYWLYYSVSSFGSNTSAIGLASSTSISSGG----WKDEGLVIRSTSSNN-YNAIDPELTFDKDGNPWLAFGSFWSGIKLTKLDKSTMKPT--GSLYSIAARPNN---------GGALEAPTLTYQNGYYYLMVSFDKCCDGVNSTYKIAYGRSKSITGPYLDKSGKSMLEGGGTILDSGNDQWKGPGGQDIVN---GNILVRHAYDANDNGIPKLLINDLNWS-SGWPSY 1GYD Chain:B ((36-332)) -----------------------------------------VHDPVMTREGDTWYLFSTG----PGITIYSSKDRVNWRYSDRAFATEPTWAKRVSPSFDGHLWAPDIYQHKGLFYLYYSVSAFGKNTSAIGVTVNKTLNPASPDYRWEDKGIVIESVPQRDLWNAIDPAIIADDHGQVWMSFGSFWGGLKLFKLNDDLTRPAEPQEWHSIAKLERSVLMDDSQAGSAQIEAPFILRKGDYYYLFASWGLCCRKGDSTYHLVVGRSKQVTGPYLDKTGRDMNQGGGSLLIKGNKRWVGLGHNSAYTWDGKDYLVLHAYEAADNYLQKLKILNLHWDGEGWPQ-

General information: TITO was launched using: RESULT: Template: 1GYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1609 315 0.20 1.14 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 0.20 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_1GYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GYD-query.scw PDB file : Tito_Scwrl_1GYD.pdb: