Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNDILEFNKTFTEQREYEKYQTSKFPDKKMAILSCMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFGSIMRSILVAVYELNADEVCVIGHHDCGMSKISSKSMLEKIKARGIPEERIETIKYSGVDFDQWFKSFDSVEASVKDSVDVIKHHPLFPENVPVHGLVIDPKTGKLDLIVNGYNN 4YF5 Chain:A ((35-168)) ----------------------------PSKHIAIVACMDARLD--VYRMLGIKEGEAHVIRNAGCVVTD---DVIRSLAISQRLLGTREIILLHHTDCGMLTFTDDDF-----KRAIQDE-------TGIRPTWSPESYPDAVEDVRQSLRRIEVNPFVTKHTSLRGFVFDVATGKLN--------

General information: TITO was launched using: RESULT: Template: 4YF5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 592 -90020 -152.06 -671.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -152.06 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_4YF5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YF5-query.scw PDB file : Tito_Scwrl_4YF5.pdb: