Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYECIQDIFGGLKNPSVKDLATSLKQIPNAAKLSQPYIKEPDQYAYGRNAIYRNN-ELEIIVINIPPNKETTVHDHGQSIGCAMVLEGKLLNSIY----RSTGEHAELSNSYFVHEGECLISTKGL-IHKMSNPT-SERMVSLHVYSPPLEDMTVFEEQKEVLENS 4XFG Chain:A ((54-170)) -------------------------------------------DQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHAFLKLLQGNLKETLFDWPDKKSNEMIKKSERT-LRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQR-------

General information: TITO was launched using: RESULT: Template: 4XFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 484 8550 17.67 77.73 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 17.67 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_4XFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XFG-query.scw PDB file : Tito_Scwrl_4XFG.pdb: