Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILI-CPSD-QAPVKMRIFNNDGSEGKNCGNGLRCVAKYAYEHKLVEETSFLIETLSGLVKAEVQVENGKVNVVTVDMGEPRLTKSELPML-DGGEEHTINETMAFGEVELTGTAVSMGNPHIVFPIADIEQAPLTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLNGVSKRNQDITVHLAGGDLVINWKDNGH-VMMTGPAETVCEGVYFL 2GKJ Chain:A ((2-274)) ----QFSKMHGLGNDFVVVDGVTQNV--FFTPETIRRLANRHCGIGFDQLLIVEAPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDISVSTQKGNMVLTVKDDNQ----IRVNMGEPIWEPAKIPFTANKFEKNYILRTDI--QTVLCG-AVSMGNPHCVVQVDDIQTANVEQLGPLLESHERFPERVNAGFMQIINKEHIKLRVYERGAGETQACGSGACAAVAVGIMQGL--LNNNVQVDLPGGSLMIEWNGVGHPLYMTGEATHIYDGFITL

General information: TITO was launched using: RESULT: Template: 2GKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1712 -41054 -23.98 -152.62 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -23.98 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_2GKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GKJ-query.scw PDB file : Tito_Scwrl_2GKJ.pdb: