Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPMIEFCVSNLA-HGSQEARAILE-KDPN--LDVLEYGCLSYCGTCMESLFALVNGEVVM------GETPAELVENIYTFIEENPMF 3DEX Chain:A ((14-89)) -RVQIEYCTQCRWLPRAAWLAQELLTTFETELTELALKP-----G-TGGVFVVRVDDEVVWDRREQGFPEPTAVKRLVRDRVA-----

General information: TITO was launched using: RESULT: Template: 3DEX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 237 -5591 -23.59 -84.71 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -23.59 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_3DEX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DEX-query.scw PDB file : Tito_Scwrl_3DEX.pdb: