Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGNKDAIFKALGDSTRRLILDELSERNELTLYELTIRLITKYDLSITRQAIAKHLSVLEDAGLVTSKRKGKYRVLIFNNEPLKNLLKGWIE 2M30 Chain:A ((19-96)) -------IFKALGDYNRIRIMELLS------VSEASVGHIS-HQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIH

General information: TITO was launched using: RESULT: Template: 2M30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 277 -54972 -198.45 -704.76 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -198.45 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_2M30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M30-query.scw PDB file : Tito_Scwrl_2M30.pdb: