Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRLFIAEDQRMLLGALGSLLDLEEDMTVIGQALNGEEALHAILKLEPDVCIMDIEMPVRSGLNVAEELMKKGCVSKVIILTTFARPGYFERAVKAGAHGYLLKDGEIDDLADAIRKCVKGKRVFSPELTFNM-----MRDE--NPLTVREQEILRLAALGKTTKDITLELYLSQGTVRNYISEIIQKLNAKNRTEAASIAEEKGWI 4IF4 Chain:A ((2-207)) -IKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIAKGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLI

General information: TITO was launched using: RESULT: Template: 4IF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 898 -82916 -92.33 -416.66 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -92.33 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.631

(partial model without unconserved sides chains): PDB file : Tito_4IF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IF4-query.scw PDB file : Tito_Scwrl_4IF4.pdb: