Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELFSVPYFIENLKQHIE---MNQSEDKIHAMNSYYRSVVSTLVQDQL-----------TKNAVVLKRIQHLDEAYNKVKRGESK 4CBE Chain:A ((140-217)) ----FVDSKFVEASKKFVNKAKEFVEENDLIALECIVKTI-GDMVNDREINSRSRYNNFYKKEADFLGAAVELEGAYKAIKQT---

General information: TITO was launched using: RESULT: Template: 4CBE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 159 12656 79.59 197.74 target 2D structure prediction score : 0.89 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 79.59 2D Compatibility (Sec. Struct. Predict.) : 0.89 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_4CBE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CBE-query.scw PDB file : Tito_Scwrl_4CBE.pdb: