Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKRIIPCLDVKEGRVVKGIQFLELKDAGDPVELAEVYDREGADELVFLDISASHEGRKTMVDVVEQVAAKLAIPFTVGGGINQLSDMKTILRAGADKVSVNTAAVLRPELITEGAEFFGSQCIVLAIDAKYDKESDTYKVYTHGGRKKTDWEVTAWAKEGVKRGAGEILLTSMDSDGEKKGFDHTLTKLVSEAVPVPVIASGGAGNAQHMLEAFTKGEADAALAASIFHYKETSIKEVKSYLKEYGVNVR 2RKX Chain:A ((1-249)) MLAKRIIAALIVKDGRVVKGSNFENLRDSGDPVELGKFYSEIGIDELSFWDITASVEKRKTMLELVEKVAEQIDIPFTVGGGIHDFETASELILRGADKVEINTAAVENPSLITQIAQTFGSQAVVVYIAAK--RVDGEFMVFTYSGKKNTGILLRDWVVEVEKRGAGEIVLGSIDRLGTKSGYDTEMIRFVRPLTTLPIIAHGGAGKMEHFLEAFLAG-ADAAKANSVFHFREIDVRELKEYLKKHGVNVR

General information: TITO was launched using: RESULT: Template: 2RKX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1443 -143231 -99.26 -575.22 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -99.26 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_2RKX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RKX-query.scw PDB file : Tito_Scwrl_2RKX.pdb: