Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNRLFRVCFLAALIMAFTLPNSVYAQKPIFKEVSVHDPSIIETNGTFYVF--GSHLASAKSNDLMQWQQLTTSVSNDNPLIPNVYEELKETFEWAQSDTLWAADVTQLADGKYYMYYNACRGDSPRSAMGVAVADNIEG-----PYKNKGIFLKSGMEGTSSDGTPYDATKHPNVVDPHTFFDKDGKLWMVYGSYSGGIFILEMNPKTGFPLPGQGYGKKLL-----GGNHSRIEGPYVLYNPDTQYYYLYLSYG----GLDATGGYNIRVARSKKPDGPYYDAEGNPMLDVRGKGGTFFDDRSIEPYGVKLMGSYTFETENEKGTGYVSPGHNSAYYDEKTGRSYLIFHTRFPGRGEEHEVRVHQLFMNKDGWPVAAPYRYAGETLKEVKQKDITGTYKLIQHGKDISADIKQTINIQLNKNHTISGEMTGTWRKTGKNTADITLAGKKYNGVFLRQWDSVREKNVMTFSVLNTSGEAVWGSK 3CU9 Chain:A ((21-310)) --------------------------------DLWAHDPVIAKEGSRWYVFHTGSGIQIKTSEDGVHWENMGWVF----PSLPDWYKQYVPE---KDEDHLWAPDIC-FYNGIYYLYYSVSTFGKNTSVIGLATNQTLDPRDPDYEWKDMGPVIHS----TASDNY--------NAIDPNVVFDQEGQPWLSFGSFWSGIQLIQLDTETMKP----AAQAELLTIASRGEEPNAIEAPFIVCR--NGYYYLFVSFDFCCRGIEST--YKIAVGRSKDITGPYVDKNGVSMMQ---GGGTILD---------------------EGNDRWIGPGHCAVYFSGVS--AILVNHAYDALKNGEPTLQIRPLYWDDEGWP-------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3CU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1597 -2868 -1.80 -10.47 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -1.80 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_3CU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CU9-query.scw PDB file : Tito_Scwrl_3CU9.pdb: