Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAMVGALIEDMAITVPVVLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADIT---ECERIVKETNIDALAAALGSVHG--KYQGEPNLGFKEMEAISRMTDIPLVLHGAS---------------------GIPQDQIKKAITLGHAKININTECMVAWTDETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG 3C4U Chain:A ((2-306)) --LVKGNEILLKAHKEGYGVGAFNFVNFEMLNAIFEAGNEENSPLFIQASEGAIKYMG-IDMAVGMVKIMCERYP-HIPVALHLDHGTTFESCEKAVKAGFTSVMIDASHHAFEENLELTSKVVKMAHNAGVSVEAELGR---------------VLVNPKEAEQFVKESQVDYLAPAIGTSHGAFKFKGEPKLDFERLQEVKRLTNIPLVLHGASAIPDNVRKSYLDAGGDLKGSKGVPFEFLQESVKGGINKVNTDTDLRIAFIAEVRKVANEDKSQFDLRKFFSPAQLALKNVVKERMKLLGSANK-------

General information: TITO was launched using: RESULT: Template: 3C4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1407 -92659 -65.86 -350.98 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -65.86 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_3C4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C4U-query.scw PDB file : Tito_Scwrl_3C4U.pdb: