Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKGLGKGINALFNQVDLSEETVEEIKIADLRPNPYQPRKHFDDEALAELKESVLQHGILQPLIVRKSLKGYDIVAGERRFRAAKLAGLDTVPAIVRELSEALMREIALLENLQREDLSPLEEAQAYDSLLKHLDLTQEQLAKRLGKSRPHIANHLRLLTLPENIQQLIAEGTLSMGHGRTLLGLKNKNKLEPLVQKVIAEQLNVRQLEQLIQQLNQNVPRETKKKEPVKDAVLKERESYLQNYFGTTVNIKRQKKKGKIEIEFFSNEDLDRILELLSERES 1VZ0 Chain:H ((23-210)) ----------------------VVRLPLASIRPNPRQPRKRFAEESLKELADSIREKGLLQPLLVRPQGDGYELVAGERRYRAALMAGLQEVPAVVKDLTDREALELALVENLQREDLSPVEEARGYQALL-EMGLTQEEVARRVGKARSTVANALRLLQLPPEALEALERGEITAGHARALLMLEPEDRLWGL-KEILEKGLSVRQAEALR----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 614 -77918 -126.90 -414.45 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain H : 0.79 3D Compatibility (PKB) : -126.90 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.703

(partial model without unconserved sides chains): PDB file : Tito_1VZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VZ0-query.scw PDB file : Tito_Scwrl_1VZ0.pdb: