Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLTDKTVLITGGASGIGYAAVQAFLNQQANVVV----ADIDEAQGEAMIRKENNDRLHFVHTDITDEPACQNAIRSAVDKFGGLDVLINNAGIEIVAPIHEMELSNWNKVLNVNLTGMFLMSKHALKYMLKSGKG-NIINTCSVGGVVAWPDIPAYNASKGGVLQLTRSMAVDYA--KHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVNPLLRLGKPEEIANVMLFLASDLSSYMTGSAITADGGYTAQ 4RF2 Chain:B ((24-272)) -RLKGKVAIVTGGTLGIGLAIADKFVEEGAKVVITGRHADVGEKAAKSI---GGTDVIRFVQHDASDEAGWTKLFDTTEEAFGPVTTVVNNAGIAVSKSVEDTTTEEWRKLLSVNLDGVFFGTRLGIQRMKNKGLGASIINMSSIEGFVGDPTLGAYNASKGAVRIMSKSAALDCALKDYDVRVNTVHPGYIKTPL-----VDDLEGA-EEMMSQRTK-TPMGHIGEPNDIAWICVYLASDESKFATGAEFVVDGGYTAQ

General information: TITO was launched using: RESULT: Template: 4RF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1385 -25750 -18.59 -106.40 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -18.59 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_4RF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RF2-query.scw PDB file : Tito_Scwrl_4RF2.pdb: