Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAKLGKSDLQVFPIGLGTNAVGGHNLYPNLNEETGKELVREAIRNGVTMLDTAYIYGIGRSEELIGEVLREFNREDVVIATKAAHRKQGNDFVFDNSPDFLKKSVDESLKRLNTDYIDLFYIHFPDEHTPKDEAVNALNEMKKAGKIRSIGVSNFSLEQLKEANK---DGLVDVLQGEYNLLNREAEKTFFPYTKEHNISFIPYFPLVSGLLAGKYTEDTTFPEGDLRNEQEHFKGERFKENIRKVNKLAPIAEKHNVDIPHIVLAWYLARPEIDILIPGAKRADQLIDNIKTADVTLSQEDISFIDKLFA 5C7H Chain:A ((5-269)) IPTIAFP-DGRKVPALGQGTWRMGEN-RA---KTADEVRSLQTGLDLGMTLIDTAEMYGDGAAERIVGEAIKGR-RDEAFVVSKVLP--------SNASRAGTVAACERSLRNLGIDCVDLYLLHWRGGY-PLAETVAAFEELKKAGKIRAWGVSNFDVDDMEELSAVPDGGNVAANQVLYNLARRGIEFDLLPRCRAQGVPVMAYSPLDEGRLLHD-------------------------------ADLIHIAKAHQATPAQVALAFLKTCSGV-ISIPKTGSPERARENRDAMDIHLTTENLAELDRHF-

General information: TITO was launched using: RESULT: Template: 5C7H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1463 -58036 -39.67 -221.51 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -39.67 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_5C7H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C7H-query.scw PDB file : Tito_Scwrl_5C7H.pdb: