Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKDVTKVCIGKIEELPEQLGKTVYIEDKELAVFKLSDGSIRAIENRCPHKGGVLAEGIVSG----QYVFCPMHDWKISLEDGIVQE-P---------DHGCVKTYETLIEGEHVYLVY 3D89 Chain:A ((14-132)) ----KYTSVCVGREEDIRKSERMTAVVHDREVVIFYH-KGEYHAMDIRCYHSGGPLHLGEIEDFNGQSCIVCPWHKYKITLATGEGLYQSINPKPKWCSKGVKQRIHTVKVDNGNIYVT-

General information: TITO was launched using: RESULT: Template: 3D89.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 466 -73343 -157.39 -733.43 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -157.39 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_3D89.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D89-query.scw PDB file : Tito_Scwrl_3D89.pdb: