Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 4UOF Chain:B ((4-150)) --ERTFIAVKPDGVQRGLIGEIIKRFEAKGFKLAGMKYIQASEDLLKQHYIDLADKPFYPGLCKYMSSGPVVAMCWEGTGVVKTARVMMGETRPADSKPGTIRGDFCIEVGRNIIHGSDSVESANKEIALWFKPEELVSWTQTNESWIY

General information: TITO was launched using: RESULT: Template: 4UOF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 669 -70112 -104.80 -476.95 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : -104.80 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_4UOF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UOF-query.scw PDB file : Tito_Scwrl_4UOF.pdb: