Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMALTGNKAVVYKGKGT-VAVEDIGYPELILRDGPGVPKANVNRKCEHGVILKVITTNICGSDQHMVRGRTT-APEGLVLGHEITGEVIETGRDVEFIKKGDIVSVPFNIACGRCVMCKTQKTHVCLNVNPDRPGSAYGY---VDM---------GGWVGGQSEYVMVPYADFQLLVFPDKEQALEKILDLTMLSDIFPTGFHGAYT-AGVQTGSTVYIAGAGPVGLAAAHSAQLLGASTVIVGDLNEDRLAQARSFGC-ETVNVQKHD-RLGEQIEQILGEPTVDAAVDCVGFEASGHGNQGEAPAAVLNSIMDVTQVG-GSLGIPGLYVTEDPGAKDADAKTGSLKIRFGLGWAKAHTFVTG-QT--PAMTYNRNLMKAILSGRAQIAKAVNATVISLDDAPKGYSDFDKGAAKKFVIDPHGTLK 3UKO Chain:A ((4-377)) ---GQVITCKAAVAYEPNKPLVIEDVQVAPPQ----------------AGEVRIKILYTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSATGVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVS--VAKIDPT----APLDKVCLLGCGVPTGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGVNEFVNPKDHDKPIQEVIVDLT-DGGVDYSFECIGNV------------SVMRAALECCHKGWGTSVIVGVAASGQ-----------EISTRPFQ-LVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLT-LGEINKAFDLLHEGTCLRCVLDTS----

General information: TITO was launched using: RESULT: Template: 3UKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2204 -30358 -13.77 -86.00 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -13.77 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_3UKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UKO-query.scw PDB file : Tito_Scwrl_3UKO.pdb: