Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASHLVLYTGAKMPILGLGTWKSP-PGKVTEAVKVAIDLGYRHIDCAHVYQNENEVGLGLQEKLQGQVVKREDLFIVSKLWCTDHEKNLVKGACQTTLRDLKLDYLDLYLIHWPTGFKPGKDPFPLDGDGNVVPDESDFVETWEAMEELVDEGLVKAIGVSNFNHLQVEKILNKPGLKYKPAVNQIEVHPYLTQEKLIEYCKSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKYNKTTAQVLIRFPMQRNLIVIPKSVTPERIAENFQVFDFELSPEDMNTLLSYNRNWRVCALMSCASHKDYPFHEEY 4GIE Chain:A ((14-279)) --NCVTLHNSVRMPQLGLGVWRAQDGAETANAVRWAIEAGYRHIDTAYIYSNERGVGQGIRES----GVPREEVWVTTKVWNSDQGYEKTLAAFERSRELLGLEYIDLYLIHWPGKK--------------------KFVDTWKALEKLYEEKKVRAIGVSNFEPHHLTELFKSCK--IRPMVNQVELHPLFQQRTLREFCKQHNIAITAWSPLGSGE-------EAGILKNHVLGEIAKKHNKSPAQVVIRWDIQHGIVTIPKSTNKGRIQENFNVWDFKLTEEEMRQIDELNEDKRIGA----------------

General information: TITO was launched using: RESULT: Template: 4GIE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -172565 for 2276 contacts (-75.8/contact) + 2D Compatibility (PS) -28734 + (NN) -13989 + (LL) 1548 1D Compatibility (HY) -22000 + (ID) 5500 Total energy: -241240.0 ( -105.99 by residue) QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_4GIE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GIE-query.scw PDB file : Tito_Scwrl_4GIE.pdb: