Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNNSGSKAELVVGGKYKLVRKIGSGSFGDVYLGITTTNGEDVAVKLESQKVKHPQLLYESKLYTILQGGVGIPHMHWYGQEKDNNVLVMDLLGPSLEDLFNFC-SRRFTMKTVLMLADQMISRIEYVHTKNFLHRDIKPDNFLMGT-GRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKHLIGTVRYASINAHLGIEQSRRDDMESLGYVFMYFNRTSLPWQGLRAMTKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEVPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTQTGKQTEKNKNNVKDN 4NFN Chain:A ((14-301)) -------PANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTT-GDVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKD---KEQVGMIKEKYE---HRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKAG-----------------------------------

General information: TITO was launched using: RESULT: Template: 4NFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -141409 for 2278 contacts (-62.1/contact) + 2D Compatibility (PS) -30051 + (NN) -9138 + (LL) 2104 1D Compatibility (HY) -21600 + (ID) 5000 Total energy: -205094.0 ( -90.03 by residue) QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_4NFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NFN-query.scw PDB file : Tito_Scwrl_4NFN.pdb: