Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKMSLFQNMKSKLLPIAAVSVLTAGIFAGAELQQTEKASAKKQDKAEIRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNNPKLTEFDR-NLTGMMMTHPDDPDYN----ITDSAAAGTALATGVKTYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHATPAAYGAHNESRKNMDQIANSY--------------MDDKIKGKHKIDVLLGGGKSYFNRK-------DRNLTKEFKQ-AGYSYVTTKQALKKNKD----QQVLGLFADGGLAKAL-----------------------DRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDKHTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSKGASKAIIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK 1ED9 Chain:A ((1-449)) TPEMPVLENRAAQ------GDITAPGGARRLTGDQTAALRDS-LSDKPAKNIILLIGDGMGDSEITAARNYAE----GAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNA-RADVTLGGGAKTFAETATAGEWQGKTLREQAQAR-GYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVR-WLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAP--------------DTKAPGLTQALNTKDG-AVMVMSYGNSE------------------------------------EDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK-

General information: TITO was launched using: RESULT: Template: 1ED9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2508 -61497 -24.52 -155.69 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -24.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_1ED9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ED9-query.scw PDB file : Tito_Scwrl_1ED9.pdb: