Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTVTQTSLYGKDLLTLKDLSEEDINALLAEAGELKQNKIQPIFHGKTLAMIFEKSSTRTRVSFEAGMAQLGGSALFLSQ-KD-----LQLGR---GET---VADTAKVLSGYVDAIMIRTF----------EHEKVEELAKEADIPVINGLTDKYHPCQALADLLTIKE------IKGKLKGVKVAYIGDGNN--VAHSLMIGCAKMGCDISIASPKGYEVLDEA-AEAAKTYALQSGSSVTLTDDPIEAVKDADVIYS------DVFTSMGQEAEEQERLAVFAPYQVNAALVSHAKPDYTFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKAILYKGESSKNC 3M4N Chain:A ((24-354)) -----------KHFLNTQDWSRAELDALLTQAALFKRNKLGSELKGKSIALVFFNPSMRTRTSFELGAFQLGGHAVVLQPGKDAWPIEFNLGTVMDGDTEEHIAEVARVLGRYVDLIGVRAFPKFVDWSKDREDQVLKSFAKYSPVPVIN-METITHPCQELAHALALQEHFGTPDLRGKKYVLTWTYHPKPLNTAVANSALTIATRMGMDVTLLCPTPDYILDERYMDWAAQNVAESGGSLQVSHDIDSAYAGADVVYAKSWGALPFFGNWEPEKPIRDQYQHFIVDERKMALTNNG----VFSHCLPLRRNVAATDAVMDSPNCIAIDEAENRLHVQKAIMAALV---------

General information: TITO was launched using: RESULT: Template: 3M4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1601 -165594 -103.43 -563.24 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -103.43 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3M4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M4N-query.scw PDB file : Tito_Scwrl_3M4N.pdb: