Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMMDANEIISFIQNSTKSTPVKVYVKGELEGINFGESAKAFINGNTGVVFGEWSEIQTAIEENQSKIEDYVVENDRRNSAIPMLDLKNIKARIEPGAIIRDQVEIGDNAVIMMGASINIGSVIGEGTMIDMNVVLGGRATVGKNCHIGAGSVLAGVIEPPSAKPVVIEDDVVIGANAVVLEGVTVGKGAVVAAGAIVVN---------------DVEPYTVVAG--TPAKKI---------------KDIDEKTKGKTEIKQELRQL 3EG4 Chain:A ((86-284)) ---------------------------------------------QSS-WWDKVPSKFDGWTANEFEKAGF---------------------RAVPNCIVRHSAYIAPNAILMPS-FVNLGAYVDKGAMIDTWATVGSCAQIGKNVHLSGGVGIGGVLEPMQAGPTIIEDNCFIGARSEVVEGCIVREGSVLGMGVFIGKSTKIVDRATGEVFYGEVPPYSVVVAGTMPGKNVPGENWGPSLYCAVIVKRADEKTRSKTSINELLRD-

General information: TITO was launched using: RESULT: Template: 3EG4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 900 -151707 -168.56 -908.42 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -168.56 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_3EG4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EG4-query.scw PDB file : Tito_Scwrl_3EG4.pdb: