Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGMFFCARAVVPFMKKSKDGAIVNVGSIAGITGAGSSMPYAVSKSAVHGLTKSLAHALAPEIRVSGVAPGAVAT---RWWAGRE--------EKMKSMIG-SLLLQCIAEPDDVAKLICSLIEQES--LTGQIITIDSGQTL 2DTD Chain:A ((108-248)) --GYYYASKFAIPYMIRSRDPSIVNISSVQASIITKNASAYVTSKHAVIGLTKSIALDYAPLLRCNAVCPATIDTPLVRKAAELEVGSDPMRIEKKISEWGHEHPMQRIGKPQEVASAVAFLASREASFITGTCLYVDGGLSI

General information: TITO was launched using: RESULT: Template: 2DTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 403 -31517 -78.21 -248.17 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -78.21 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_2DTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DTD-query.scw PDB file : Tito_Scwrl_2DTD.pdb: