Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEPNLRGNGKVALVTGAARGIGLGISAWLIAEGWQVVLADNDRERGARVAEALGEHAWFVAMDVAQEGQVAMSVAEVLGQFGRLDGLVCNAAIANPRNTPLEALSLGEWTRTLAVNLTGPMLLAKYCTPYLR-AHNGAIVNIASTRAHQSEPDSEAYAASKGGLLALTHALAASLGPD-IRVNALSPGWIDTREAAEREAAPLTELDHDQHLVGRVGTVEDVASLVAWLLSEDAGFVTGQEFLVDGGMTRKMIYLD 2HSD Chain:A ((4-242)) --------SGKTVIITGGARGLGAEAARQAVAAGARVVLADVLDEEGAATARELGDAARYQHLDVTIEEDWQRVVAYAREEFGSVDGLVNNAGIST--GMFLETESVERFRKVVEINLTGVFIGMKTVIPAMKDAGGGSIVNISSAAGLMGLALTSSYGASKWGVRGLSKLAAVELGTDRIRVNSVHPGMTYTPMTAE-TGIRQGEGNYPNTPMGRVGEPGEIAGAVVKLLSDTSSYVTGAELAVDGGWT-------

General information: TITO was launched using: RESULT: Template: 2HSD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -156920 for 2114 contacts (-74.2/contact) + 2D Compatibility (PS) -25731 + (NN) -8900 + (LL) 1228 1D Compatibility (HY) -9200 + (ID) 4500 Total energy: -204023.0 ( -96.51 by residue) QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_2HSD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HSD-query.scw PDB file : Tito_Scwrl_2HSD.pdb: