Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQVYIIHGYRASSTNHWFPWLKKRLLADGVQADILNMPNPLQP-----------RLEDWLDTLSLYQHTLH-ENTYLVAHSLGCPAILRFLEHLQLRKQLGGIILVSGFAKSLPT--------L--------QMLD----E----F----------------TQ------GS-----F-------DH-QKIIESAKHRAVIASKDDQIVPFSFSKDLAQQID-AALYEVQHGGHFLEDEGFTSLPIVYDVLTSYFSKETR 1WOM Chain:A ((20-269)) -KASIMFAPGFGCDQ-SVWNA-VAPAFE-EDHRVILFDYVGSGHSDLRAYDLNRYQTLDGYAQDVLDVCEALDLKETVFVGHSVGALIGMLASIRR--PELFSHLVMVGPSPCYLNDPPEYYGGFEEEQLLGLLEMMEKNYIGWATVFAATVLNQPDRPEIKEELESRFCSTDPVIARQFAKAAFFSDHREDLSKVTVPSLILQCADDIIAPATVGKYMHQHLPYSSLKQMEARGHCPHMS---HPDETIQLIGDYLKA---

General information: TITO was launched using: RESULT: Template: 1WOM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 915 -132564 -144.88 -744.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -144.88 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_1WOM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WOM-query.scw PDB file : Tito_Scwrl_1WOM.pdb: