Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQEKAIIIGGGPCGLSAAIHLKQIGIDALVIEKGNVVNSIYNYPTHQT-FFSSSEKLEIGDVAFITEN-R-KPVRIQALSYYREVVKRKNIRVNAFEMVRKVTKTQNNTFVIETSKETYTTPYCIIATGYYDHPNYMGVPGEDLP--KVFH--YFKEGHPYFDKDVVVIGGKNSSVDAALELVKSGARVTVLYRGNEYSPSIKP------WILPEFEAL------------------------VRNGTIRMEFGACVEKITENEVVFRSGEKELITIKNDFVFAMTGYHPDHQFLEKIGVEIDKETGRPFFNEETMETNVEGVFIAGVI--AAGNNA-NEIFIENGRFHGGHIAAEIAKRENH 4USR Chain:A ((6-355)) -PILDVIVIGGGQAALTTAYFLRRTSLSYLLLDEQPGPGGAWLHAWDSLRLFSPAAWSSIAGWPMPSPTEPGNPTRNDVIDYLRRYEDRYQFPIQRPVRVDTVTRL-DDLWRVQAGDQQWLARAVISATGTWSKPFIPPYEGRELFQGAQIHSAHYRTPAPFAGKRVMVVGGGNSGAQVLAELSSVSETLWITQEPPAFLPDEVDGRVLFERATARWKAQQEGRSIDEPAGGFGDIVMVPPVREARERGVLVAE-RPFARFTETGVEWADG----RRENLDAVIWCSGFRPALDHLRELGVVEA--DGKVQVED-TRVVKQPNLWLVGYGDWTGMASATLIGVTRTARSTADQVVQALTA----

General information: TITO was launched using: RESULT: Template: 4USR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1500 -7384 -4.92 -23.82 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -4.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_4USR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4USR-query.scw PDB file : Tito_Scwrl_4USR.pdb: