Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHSQEKIALITGASSQGDIGTAICRKLASQGIHIFFTHWNSDTAWIEEFQQEILRMGVRCEAMKIDLSDAHAAFTIHEKISDKLGYPSILINNAAHSASDNYVSLDAKSLDEHYAVNMRSNFLLCVEFARRFKKSNLISGRIINMTSGQDLGPL--PGELAYAATKGAISAFTRSLSQELAPLGITVNAVNPGPTDSTWMTDEI----RNFLSPKFPMGRIGTPDDAARMIAFLASDEAEWITGQIIHSEGGFIRG 4BNX Chain:A ((26-266)) ----QGKVALVTGASR--GIGQAIALELGRLGAVVIGTATSASGA--EKIAETLKANGVEGAGLVLDVSSDESVAATLEHIQQHLGQPLIVVNNA---------RMKDDEWFDVVNTNLNSLYRLSKAVLRGMTKARW--GRIINI--GSVVGAMGNAGQTNYAAAKAGLEGFTRALAREVGSRAITVNAVAPGFID-TDMTRELPEAQREALLGQIPLGRLGQAEEIAKVVGFLASDGAAYVTGATVPVNGGM---

General information: TITO was launched using: RESULT: Template: 4BNX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1225 -144216 -117.73 -638.12 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -117.73 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_4BNX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BNX-query.scw PDB file : Tito_Scwrl_4BNX.pdb: