Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENVFDYEDIQLIPAKCIVNSRSECDTSVRLGGH-TFKLPVVPANMQTIIDEKLAISLAENGYFYVMHR-FEPETRID-FIKDMNARGLFSSISVGVKDEEYEFVRQLAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASK---PIIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHEESPGQTIEKDGKLYKEYFGSAS------EFQKGEKKN---VEGKKMHVAHKGSIKDTLIEMEQDLQSSISYAGGTKLNAI-RNVDYVIVKNSIFNGDKY 4QJ1 Chain:D ((13-340)) ----LTFEDILLVPNYSEVLPR-EVSLETKLTKNVSLKIPLISSAMDTVTEHLMAVGMARLGGIGIIHKNMDMESQVNEVLKVKNSGGLRVGAAIGVN--EIERAKLLVEAGV--DVIVLDSAHGHSLNIIRTLKEIKSKM-NIDVIVGNVVTEEATKELIENGADGIKVGIGPGSICTTRIVAGVGV--PQITAIEKCSSVASKFGIPIIADGGIRYSGDIGKALAVGASSVMIGSILAGTEESPGEKELIGDTVYKYYRGMGSVGAMKS--------NKMVPEGIEGRVKYKGEMEGVVYQLVGGLRSCMGYLGSASIEELWKKSSYVEITTS-------

General information: TITO was launched using: RESULT: Template: 4QJ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1854 -19160 -10.33 -64.08 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -10.33 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_4QJ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QJ1-query.scw PDB file : Tito_Scwrl_4QJ1.pdb: