Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTTN-GWINGFIGVLIFSGSLPATRLAVSDF---------DPLFLTVCRAAIAG-VLAGGLLLIF--RQQHPAK-RDLISLLVVA--------------FGVVIGFP-------LLTALAL-QHVTSAHAI----------VFIGLLPLATAV-FGVLRGGERPRP---------------------V-F-WIFSAA-------GSLLVAGFA---------------LI---QGGGSSPLGDA-YMLASIVVCGL--GYAEGAKLS-RRLGNWQVISWALVLSLPLMLP--------LSFFFTPDSWSAIG------------VPALLSLAYVSLFSMLI-GFVF-------------------------WYRGLAQGGIAAVGQLQL-LQPFFGLLLASVI-LHEKVGWALVAVNIAVIMCVAAARRFAK 4QT9 Chain:A ((19-432)) LSDDALMDTVQRRTFLYFWEGAEPNSGLAPERYHVDGVYPQNDANVVTSGGSGFGIMAILAGIDRGYVTREEGLARMERIVSFLEKADRFHGAYPHWWYGDTGKVKPFGQKDNGGDLVETAFLMQGLLAVHQYYANGNDKEKAIAQRIDRLWREVDWDWYRKGGQNVLYWHWSPTYGWEMDFPIHGYNECMIMYILAAASPTHGVPAAVYHDGWAQNGAIVSPHKVEGIELHLRYQGTEAGPLFWAQYSFLGLDPVGLKDEYCPSYFHEMR---NLTLVNRAYCIRNPKHYKGFGPDCWGLTASYSVDGYAAHSPNEQDDKGVISPTAALSSIVYTPEYSLQVMRHLYNMGDKVFGPFGFYDAFSETDNWYPQRYLAIDQGPIA------VMIENYRTGLLWKLFMSHPDVQAGLTKLGFNTN-----------

General information: TITO was launched using: RESULT: Template: 4QT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1262 -206477 -163.61 -773.32 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -163.61 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.151

(partial model without unconserved sides chains): PDB file : Tito_4QT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QT9-query.scw PDB file : Tito_Scwrl_4QT9.pdb: