Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGKNGLELLTELRAQNEAVDVIVISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRKQKVYSTHRNMSQKELDAELFQKKEATEKVQLPKGLTKSTLKLIWSSIQSFENESFTTEDLAKHT---EISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL 1P2F Chain:A ((2-198)) MWKIAVVDDDKNILKKVSEKLQQL-GR-V-KTFLTGEDFLN--DEEAFHVVVLDVMLPDYSGYEICRMIKETRPETWVILLTLLSDDESVLKGFEAGADDYVTKPFNPEILLARVKRFLEREKKGLY--DFGDLKID---ATGFTV-FLKGKRIHLPKKE-FEILLFLAENAGKVVTREKLLETFWEDPVSPRVVDTVIKRIRKAIEDD-----------------------------

General information: TITO was launched using: RESULT: Template: 1P2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 887 -106088 -119.60 -546.84 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -119.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_1P2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P2F-query.scw PDB file : Tito_Scwrl_1P2F.pdb: