Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLIEREKTVYYKEKPDPSSLGFGQYFTDYMFVMDYEEGIGWHHPRIAPYAPLTLDPSSSVFHYGQAVFEGLKAYRTDDGRVLLFRPDQNIKRLNRSCE--RMSMPPLDEELVLEALTQLVELEKDWVPKEKGTSLYIRPFVIATEPSLGVK--ASRSYTFMIVLSPVGSYYGDDQLKPVRIYVEDEYVR-AVNGGVGFAKTAGNYAASLQAQRKANELGYDQVLWLDAIEKKYVEEVGSMNIFFVINGEAVTPALSGSI-LSGVTRASAIELIRSWGIPVREERISIDEVYAASARGELTEVFGTGTAAVVTPVGELNIHGKTVIVGDGQIGDLSKKLYETITDIQLGKVKGPFNWTVEV 1WRV Chain:C ((2-294)) -----------------------------------QIKAGLIWMNGAFVPQEEAKTSVLSHALHYGTSVFEGIRAYETAKGPA-IFRLKEHVKRFYNSAKVLRMEIPFAPEELE-EAIKEVVR-------RNGYRSCYIRPLAWMGAKALGVNPLPNNPAEVMVAAWEW----------KGARLITSSWARFPANVMPGKAKVGGNYVNSALAKMEAVAAGADEALLLD--EEGYVAEGSGENLFFVRDG--VIYALEHSVNLEGITRDSVIRIAKDLGYEVQVVRATRDQLYMAD------EVFMTGTAAEVTPVSMIDWRP----IGKGTAGPVALRLREVYLEAVTGR-----------

General information: TITO was launched using: RESULT: Template: 1WRV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1511 -140694 -93.11 -507.92 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.70 3D Compatibility (PKB) : -93.11 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1WRV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WRV-query.scw PDB file : Tito_Scwrl_1WRV.pdb: