Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPMDRQTEGQEPQHQDRQPGIESKMNPLPLSEDEDYRGSGKLKGKVAIITGGDSGIGRAAAIAFAKEGADISILYLDEHSDAEETRKRIEKENVRCLLIPGDVGDENHCEQAVQQTVDHFGKLDILVNNAAEQHPQDSILNISTEQLEKTFRTNIFSMFHMTKKALPHL--QEGCAIINTTSITAYEGDTALIDYSSTKGAIVSFTRSMAKSLADKGIRVNAVAPGPIWTPLIPATFPEEKVKQHGLDTPMGRPGQPVEHAGAYVLLASDESSYMTGQTIHVNGGRFIST 3RRO Chain:B ((10-250)) ------------------------------------------LEGKVALVTGASRGIGKAIAELLAERGAKV-IGTATSESGAQAISDYL-GDNGKGMAL--NVTNPESIEAVLKAITDEFGGVDILVNNAGITR-DNLLMRMKEEEWSDIMETNLTSIFRLSKAVLRGMMKKRQGRIINVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVTVNTVAPGFIETDMTKALNDEQRTATLA-QVPAGRLGDPREIASAVAFLASPEAAYITGETLHVNGGMYM--

General information: TITO was launched using: RESULT: Template: 3RRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1328 -121919 -91.81 -510.12 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -91.81 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_3RRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RRO-query.scw PDB file : Tito_Scwrl_3RRO.pdb: