Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVLNRRQALQRALLNGKNKQDAYHPFPWYESMRKDAP---VSFDEENQVWSVFLYDDVKKVVGDKELFSSCMPQQT-----SSIGNSIINMDPPKHTKIRSVVNKAFTPRVMKQWEPRIQEITDELIQKFQGRSE-FDLVHDFSYPLPVIVISELLGVPSAHMEQFKAWSDLLVSTPKDKSEEAEKAFLEERDKCEEELAAFFAGIIEEKRNKPEQDIISILVEAEETGEKLSGEELIPFCTLLLVAGNETTTNLISNAMYSILETPGVYEELRSHPELMPQAVEEALRFRAPAPVLR-----RIAKRDTEIGGHLIKEGDMVLAFVASANRDEAKFDRPHMFDIRRHPNPHIAFGHGIHFCLGAPLARLEANIALTSLISAFPHMECVSITPIENSVIYGLKSFRVKM 4Z5P Chain:A ((18-373)) ---------------------DGLEIHPKFAELRETDPLARVRLPYGGEGWMVTRYDDVRAANSDPRFSRAQIGEDTPRTTP----DTILSLDPPEHTRLRRLLSKAFTARRMGAMQSWLEELFAGLLDGVERTGHPADIVRDLAQPFTIAVICRLLGVPYEDRGRFQHWSEVIMSTTAYSKEEAVSA--------DASIRAYLADLVSARRAAPHDDLLGVLVSARDDDDRLTEDELITFGVTLLVAGHETSAHQLGNMVYALLTHEDQLSLLREQPELLPRAVEELLRF---VPLGNGVGNARIALEDVELSGGTVRAGEGVVAAAVNANRDPRAFDDPDRLDITREKNPHLAFGHGAHYCLGAQLARMELRVAIGGLLERFPGL-----------------------

General information: TITO was launched using: RESULT: Template: 4Z5P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1517 -193804 -127.75 -575.08 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -127.75 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_4Z5P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z5P-query.scw PDB file : Tito_Scwrl_4Z5P.pdb: