Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFKKNFLVGLSAALMSISLFSATASAASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPF-------RTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTG-TYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFSNHVNAWKSHGMNLGSNWAYQV-MATEGYQSSGSSNVTVW 1H4H Chain:D ((16-194)) ---------------------------------YEFWKDSGGSGTMILNHGGTFSAQWNNVNNILFRKGKKFNETQTHQQVGNMSINYGAN-FQPNGNAYLCVYGWTVDPLVAYYIVDSWGNWRPPGATPKGTITVDGGTYDIYETLRVNQPSIKG-IATFKQYWSVRRSKRTSG---TISVSNHFRAWENLGMNMGK--MYEVALTVEGYQSSGSANV---

General information: TITO was launched using: RESULT: Template: 1H4H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 898 31072 34.60 182.77 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : 34.60 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1H4H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H4H-query.scw PDB file : Tito_Scwrl_1H4H.pdb: