Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVMSKQMRF--DNSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNKMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSLN----GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDDLDAMSLTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYNFTIDDFKLINYKHGDKLLFEVAV 5BY6 Chain:C ((19-303)) VNQEELNYLNQLKDIIDHGVRK----------NDRTGIGTLSTFGTQSRYCLRDDIFPLLTTKRVFWRGVVEELLWFIS-GSTNAKQLSEKNVNIWDGNSSREFLDSRGLYNYEEGDLGPVYGFQWRHFGCPYSSMTADYKGKGYDQLQQCIKMIREEPESRRIIMTAWNPCDLEKVALPPCHCFVQFYVADGELSCQMYQRSADMGLGVPFNIASYSLLTRMIAHITSLKPGFFIHTIGDAHVYLTHVDALKVQMERKPRPFPKLKILRNVENIDDFRAEDFELINYKPYPKISM----

General information: TITO was launched using: RESULT: Template: 5BY6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1281 23027 17.98 87.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : 17.98 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_5BY6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BY6-query.scw PDB file : Tito_Scwrl_5BY6.pdb: