Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAG----GYEGNIDDLIIGCATPEAEQGLNMARNIGALAGLPYTVPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESMSQVPMMGHVTRPNLALAEKAPEYYMSMGH---TAEQVAKKYGVSREDQDAFAVRSHQNAAKALAEGKFKDEIVPVEVTVTEIGEDHKPM-EKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTVTAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALKLAGLQLQDIGLFELNEAFASQAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSLIHEMKRRNEQFGVVTMCIGGGMGAAGVFELC 4UBV Chain:B ((9-397)) MGYPVIVEATRSPIGK-RNGWLSGLHATELLGAVQKAVVDKAGIQSGLHAGDVEQVIGGCVTQFGEQSNNISRVAWLTAGLPEHVGATTVDCQ-GSGQQANHLIAGLIAAGAIDVGIACGIEAMSRVGL-GANAGPDRSLI-RAQSWDIDLPNQFEAAERIAKRRGITREDVDVFGLESQRRAQRAWAEGRFDREISPIQAPVLD--EQNQPTGERRLVF-RDQGLRETTMAG-LGELKPVLE-GGIHTAGTSSQISDGAAAVLWMDEAVARAHGLTPRARIVAQALVGAEPYYHLDGPVQSTAKVLEKAGMKIGDIDIVEINEAFASVVLSWARVHEPDMDRVNVNGGAIALGHPVGCTGSRLITTALHELERTDQSLALITMCAGGALSTGTIIE--

General information: TITO was launched using: RESULT: Template: 4UBV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2445 2513 1.03 6.61 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : 1.03 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_4UBV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UBV-query.scw PDB file : Tito_Scwrl_4UBV.pdb: