Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGGMALTGNKAVVYKGKGT-VAVEDIGYPELILRDGPGVPKANVNRKCEHGVILKVITTNICGSDQHMVRGRTTAPEGLVLGHEITGEVIETGRDVEFIKKGDIVSVPFNIACGRCVMCKTQKTHVCLNVNPDR-------PGSA----YGYVDMGGWVGGQSEYVMVPYADFQLLVFPDKEQALEKILDLTMLSDIFPTGFHGAYT-AGVQTGSTVYIAGAGPVGLAAAHSAQLLGASTVIVGDLNEDRLAQARSFGC-ETVNVQKHDRLGEQIEQILGEPTVDAAVDCVGFEASGHGNQGEAPAAVLNSIMDVTQVGGSLGIPGLYVTEDPGAKDADAKTGSLKIRFGLGWAKAHTFVTG-Q--TPAMTYNRNLMKAILSGRAQIAKAVNATVISLDDAPKGYSDFDKGAAKKFVIDPHGTLK 1F8F Chain:A ((4-370)) -----LKDIIAAVTPCKGADFELQALKIRQPQ----------------GDEVLVKVVATGMCHTDLIVRDQKYPVPLPAVLGHEGSGIIEAIGPNVTELQVGDHVVLSYG-YCGKCTQCNTGNPAYCSEFFGRNFSGADSEGNHALCVNDHF----FAQSSFATYALSRENN--TVKVTKD----VPIELLGPLGCGIQTGAGACINALKVTPASSFVTWGAGAVGLSALLAAKVCGASIIIAVDIVESRLELAKQLGATHVINSKTQ-DPVAAIKEITD-GGVNFALESTGSP------------EILKQGVDALGILGKIAVVGAPQLGT-----------TAQFDVNDLLLGGKTILGVVEGSGSPKKFIPELVRLYQQGKFPFDQ-LVKFYA-FDEINQAAIDSRKGITLKPIIKI-----

General information: TITO was launched using: RESULT: Template: 1F8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2066 -45511 -22.03 -132.30 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -22.03 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_1F8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F8F-query.scw PDB file : Tito_Scwrl_1F8F.pdb: