Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRDLTDDVALMRPVIDALRASGCDPDRVLVRVGLPPGGLPAGRFPHSAQNLFWKAAADECGEEHVGLHLAEHLPAFHGLLLEYLFLSSDTFGAGLRHSLRYVRLLSDTLQAQLEVEGERAVLSLGESPAINRHFPEMLAGAVIRLFGALTEGEFKPLEVQLMNETGAPMERYRAVYGCPTILGMPRYALVFDAAVLDKPSRHAAPELLRMHESLARRQLAEVERLDLVRQVRELIGELLVDGGATLEQVAARL-GMPARRLRERLAMA-GVRFNDLVTDYRCRLAKELLLKTDERIEVIVERTGFSEPSTFYRAFKRWVGETPVEFRRRGQQGRG 3OTD Chain:A ((3-269)) -KSKFEYVRDFEADDTCLAHC-----WVVVRLDGRN-------FHRFAEKHNFAKPNDSRALQLMTKCAQTVMEELEDIVIAYGQ-------------------------------SDEYSFVFKRKTNWFKRRASKFMTHVASQFASSYVFYWRDYFEDQPLLYPPG----------------------FDGRVVVYPSNQTLKDYLSWRQADCHINNLYNTVFWALIQ----------QSGLTPVQAQGRLQGTLAADKNEILFSEFNINYNNE--------------LPMYRKGTVLIWQTKPVPL-----HCDIIGDAFWKEHPEILDEDS

General information: TITO was launched using: RESULT: Template: 3OTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -71952 for 1759 contacts (-40.9/contact) + 2D Compatibility (PS) -25536 + (NN) -11417 + (LL) 7092 1D Compatibility (HY) -2000 + (ID) 1550 Total energy: -105363.0 ( -59.90 by residue) QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_3OTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OTD-query.scw PDB file : Tito_Scwrl_3OTD.pdb: