Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTTNGWINGFIGVLIFS-----GSLPATRLAVSDFDPLFLTVCRAAIAGVLAGGLLLIFRQQ---HPAKRDLISLLVVAFGVVIGFPLLTALALQHVT------SAHAIVFI------GLLPLATAVFGVLRGGERPRPVFW--------------IFSAAGSLLVAGFALIQ-------------GGGSSPLGDAYMLASIVVCGLGYAEGAKLSRRL--------GNWQVISWALVLSL------PLMLPLSFFFTPDSWSAIGVPALLSLAYVSLFSMLIGFVFWYRGLAQGGIAAV--GQLQLLQPFFGLLLASVILHEKVGWALVAVNIAVIMCVAAARRFAK--------- 1FUI Chain:A ((1-355)) MKKISLP----KIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEKLRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPTRPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLLRFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRIDQKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQGNSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVVATENDSLNGVAMLMGHQLTGT

General information: TITO was launched using: RESULT: Template: 1FUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1141 -169055 -148.16 -597.37 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -148.16 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.190

(partial model without unconserved sides chains): PDB file : Tito_1FUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FUI-query.scw PDB file : Tito_Scwrl_1FUI.pdb: