Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEVKRRNI---QDPKEVQSVISEKLVEIYNSGDE--QISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFRAGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPHEVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLFSDLVEKADD 3E70 Chain:C ((52-324)) -----------------------------------------------VDKALDELEIDLLEADVALEVVDALREKIKQKLVGKKVRIG--GKIIEEAVKEAVSEILETSRRIDLIEEIRK-AEKPYVIMFVGFNGSGKTTTIAKLANWLKNHGFSVVIAASDTFRAGAIEQLEEHAKRIGVKVIKHSYGADPAAVAYDAIQHAKARGIDVVLIDTAGRSETNRNLMDEMKKIARVT------KPNLVIFVGDALAGNAIVEQARQFNEAVKIDGIILTKLDADARGGAALSISYVIDAPILFVGVGQGYDDLRPFEKEWFLERIFG--------

General information: TITO was launched using: RESULT: Template: 3E70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1377 -171234 -124.35 -646.16 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -124.35 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_3E70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E70-query.scw PDB file : Tito_Scwrl_3E70.pdb: