Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRF--DNSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSLN----GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYDFTIDDFKLINYKHGDKLLFEVAV 5BY6 Chain:B ((19-304)) VNQEELNYLNQLKDIIDHGVRK----------NDRTGIGTLSTFGTQSRYCLRDDIFPLLTTKRVFWRGVVEELLWFIS-GSTNAKQLSEKNVNIWDGNSSREFLDSRGLYNYEEGDLGPVYGFQWRHFGCPYSSMTADYKGKGYDQLQQCIKMIREEPESRRIIMTAWNPCDLEKVALPPCHCFVQFYVADGELSCQMYQRSADMGLGVPFNIASYSLLTRMIAHITSLKPGFFIHTIGDAHVYLTHVDALKVQMERKPRPFPKLKILRNVENIDDFRAEDFELINYKPYPKISMP---

General information: TITO was launched using: RESULT: Template: 5BY6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1296 28019 21.62 105.73 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 21.62 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_5BY6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BY6-query.scw PDB file : Tito_Scwrl_5BY6.pdb: