Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDVQHIDHSFTIGKKGRENEVPVLKDVSLSVAKGEIACIVGRSGSGKSTLLNLISGYISPTKGRIVINGTDVTGFNEKEWAQFRLDHFGFIFQSFQLIPGLTTYENVEMPLALKGIKPSERKQKVQDMLKRVGLENHAAHYPN-----------ELSGGQQQRVSIARALILNPSIILADEPTGSLDSETEHEVLELIQQLNRERGITFV-----IITHDDEVASI--GHSKFQLHDGVLKGGITVEV 2HYD Chain:B ((340-552)) -IDIDHVSFQYN------DNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYDVTSGQILIDGHNIKDFLTGSLR----NQIGLVQQDNILFSD-TVKENILLG------RPTATDEEVVEAAKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSALDLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGH------------------

General information: TITO was launched using: RESULT: Template: 2HYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 941 -63528 -67.51 -325.78 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -67.51 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2HYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HYD-query.scw PDB file : Tito_Scwrl_2HYD.pdb: