Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MRRILSILVFAIMLAGCSSNASTEKQHAGGEKTVKAEPQSTSSQKDSTDDYQPNSQVTD--DRTLHKVGQTFSDDKGKAVLKDIKQVNKT-------YKIGDVELTVKEMKLIHLRPDYSMIDYFHELTHDEEFD------FVKVFVDIKNTSTKKVNVAPIALMKTNTGETFD--------------------WNKDIYLEELNGELEGGAEKSGNLGFIVNASSGHAHDK-----------AADAEKKTKEIKWIEITTSDVFDHNHKKIS------------------------------DAQKIKIKF--------- 1BO1 Chain:A ((34-416)) KLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEFLAQLKIMDYSLLVGIHDVDRAEQEEMEVEERAEDEEFDPSVDVYAMKSHESSPKKEVYFMAIIDILTAGAEISTVNPEQYSKRFNEFMSNILT

General information: TITO was launched using: RESULT: Template: 1BO1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 802 -17735 -22.11 -75.15 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -22.11 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_1BO1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BO1-query.scw PDB file : Tito_Scwrl_1BO1.pdb: